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KMID : 0381120070290040547
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Genes and Genomics
2007 Volume.29 No. 4 p.547 ~ p.555
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Differentiation of B Genome Chromosomes in Wheats Revealed by FISH Analysis Using a Fungus Derived TC/AG Microsatellite Sequence
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Lee Myung-Sook
Oh Hae-Young
Son Jae-Han
George Fedak
Kim Nam-Soo
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Abstract
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Molecular cytogenetic analysis was carried out in the ancestral genome donors to polyploid wheats, T. turgidum, and T. aestivum, with a fungus derived TC/AG microsatellite sequence, pKFJ660. The 45S rRNA gene was also used for chromosome identification in FISH analysis. The A and D genome donors, T. monococcum and T. tauchii, showed no hybridization signals with the pKFJ660. The hybridization sites were variable in the chromosomes of S genome Sitopsis species which were the putative B genome donors to the polyploid wheats. Four pairs of chromosomes showed pKFJ660 hybridization signals in T. turgidum (1B, 3B, 5B and 6B) and T. aestivum (3B, 4B, 5B, and 6B). Differences of hybridization signal intensities and FISH signal distributions were high among the S genome diploids and polyploid wheats, which hindered providing unequivocal explanations for the wheat evolution with this microsatellite sequence. With the evidences obtained, it seemed to be evident that the pKFJ660 microsatellite sequence had undergone restructuring and reorganization in its sequence along the chromosomes during wheat evolution. Since the pKFJ660 contained AluI transposon related
direct repeat, possibilities of transposon mediated genome instability by allopolyploidization were discussed.
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KEYWORD
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Triticum, FISH, wheat evolution, Sitopsis species
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